大鼠急性脑缺血再灌注后血脑屏障通透性的超微结构与血清S100B蛋
作者：吴松笛，万琪，夏峰，耿晓英，刘学东，王津存【关键词】 血脑屏障
Dynamic changes of bloodbrain barrier permeability and S100B protein level in serum after acute cerebral ischemia/reperfusion in rats
【Abstract】 AIM: To investigate the dynamic changes of the bloodbrain barrier（BBB） permeability and the S100B protein level in serum after acute cerebral ischemia/reperfusion in rats. METHODS: The middle cerebral artery occlusion/reperfusion (MCAO/R) rat model was made by the thread occlusion method; the permeability of BBB was investigated by transmission electron microscope using lanthanum nitrate as the tracer; the serum S100B protein concentrations were determined by ELISA at 0.5, 2, 6, 12, 24 and 48 h reperfusion after 2 h cerebral ischemia. RESULTS: ① Electron microscopy revealed that lanthanum granules appeared around the tight junction and in the endotheliocytes at 0.5 h areperfusion, indicating the increase of BBB permeability. More phagotrophic lanthanum granules were found in the endotheliocytes that showed significant vacuolization at 2 and 6 h. The tight junction was opened and the astrocytes were separated from the basement membrane of the capillaries. The necrosis of both neurons and astrocytes with dissolved nucleus and the disruption of the capillary basement membrane were found at 12 and 24 h. The necrosis and disaggregation of many neurons and astrocytes were evident at 48 h. A lot of lanthanum granules were shown outside the brain vessels while many endotheliocytes were broken. ② The serum S100B protein level increased significantly only in rats after cerebral ischemia/reperfusion injury, but not in the shamoperation and the nonoperation control rats (P
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