肺表面活性物质在缺血预处理肺保护中的作用
【关键词】 缺血/再灌注Role of pulmonary surfactant on lung ischemic preconditioninginduced protection against lung ischemia reperfusion injury
【Abstract】 AIM: To investigate whether the pulmonary surfactant (PS) is involved in the protective effects of lung ischemic preconditioning (IPC) against lung ischemia reperfusion (I/R) injury. METHODS: In vivo I/R injury of rabbit was induced by blocking the hilum of left lung. The arterial partial pressure of oxygen (PaO2), lung permeability index, leucocyte count and neutrophils percentage in bronchoalveolar lavage fluid (BALF) were detected as indexes of lung injury. Total phospholipid (TPL), saturated phosphatidylcholine (SatPC) and total protein (TP) in the BALF were respectively measured by Bartlett method, Mason method and Lowry method. The activity of PS was presented by the ratio of SatPC/TP and SatPC/TLP. RESULTS: I/R injury markedly reduced the arterial oxygen pressure, which increased in IPC group (P
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