血必净对大鼠急性胰腺炎保护作用机制探讨

时间:2020-10-04 09:06:03 临床医学毕业论文 我要投稿

血必净对大鼠急性胰腺炎保护作用机制探讨

【摘要】  目的 探讨血必净对大鼠急性胰腺炎的保护作用及机制。方法 SD大鼠48只, 随机分为假手术组、胰腺炎组和治疗组,每组再分为4 h、8 h亚组。假手术组仅行开关腹手术,胰腺炎组以牛磺胆酸钠造模,治疗组以血必净干预。光镜观察胰腺组织病理改变,免疫组化法测胰腺组织肿瘤坏死因子(TNF)-α、干扰素(IL)-1β。结果 治疗组胰腺组织损伤较胰腺炎组明显减轻,治疗组胰腺组织中TNF-α、IL-1β含量显著低于胰腺炎组 (P<0.01)。结论 血必净可能通过抑制TNF-α、IL-1β的产生减轻胰腺炎症。

【关键词】  血必净;胰腺炎;肿瘤坏死因子;干扰素

  Abstract: Objective To investigate the protective effect of Xuebijing (XBJ) on acute pancreatitis in rats and its possible mechanism. Methods 48 SD rats were randomized into 3 groups: sham group, pancreatitis group and XBJ group, each group sub-divided into 4h and 8h subgroups. In the sham groups, the rats only underwent opening and closure of the abdomen; the pancreatitis groups received taurocholate sodium administration to establish animal model and the treatment groups were intervened by Xuebijing. The pathologic changes of pancreatic tissues were observed by optic microscopy. The expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in pancreatic tissues were determined by immunohistochemical methods. Results The histomorphological changes of the pancreatic tissues in XBJ group were much more relieved than those in the pancreatitis group. The expressions of TNF-α and IL-1β were significantly lower in XBJ group than those in the pancreatitis group (P<0.01). Conclusion XBJ could relieve acute pancreatitis in rats by inhibiting the production of TNF-α and IL-1β.

  Key words: Xuebijing; pancreatitis; tumor necrosis factor-α; interleukin-1β

  肿瘤坏死因子α(TNF-α)、干扰素1β(IL-1β)是引起组织细胞损伤的重要细胞因子,两者均参与急性胰腺炎的病理过程。近年来已有关于血必净对血液中细胞因子影响的报道[1-2],但血必净对急性胰腺炎时胰腺组织TNF-α、IL-1β的表达有无影响尚未见报道。本实验旨在通过观察血必净注射液对大鼠胰腺组织TNF-α、IL-1β表达的影响,探讨血必净对大鼠急性胰腺炎的保护作用及可能机制。

  1 材料和方法

  1.1 动物模型与分组 将SD大鼠48只(体重200~250 g, 由徐州医学院实验动物中心提供)随机分为假手术组、胰腺炎组和治疗组(n=16),假手术组仅行开关腹手术,胰腺炎组经胰胆管逆行注射5%牛磺胆酸钠(2 ml/kg,美国Sigma公司)制作胰腺炎模型,治疗组在胰腺炎模型基础上予血必净干预。术前3天,治疗组经尾静脉注射血必净注射液4 ml·kg-1·d-1(天津红日药业股份有限公司生产);假手术组、胰腺炎组同时间点给予等量生理盐水。3组分别于制模术后4h、8 h时点处死大鼠,取胰腺标本进行检验。

  1.2 组织病理学检查 切取胰腺标本制成石蜡切片后行苏木精-伊红染色,光镜下观察胰腺组织病理学变化。

  1.3 TNF-α、IL-1β表达检测 免疫组化染色法(试剂盒均购自武汉博士德生物工程有限公司)测胰腺组织TNF-α、IL-1β表达,结果应用计算机进行图像灰度分析,以积分光密度值(D)作计量分析,每张片选4个视野。

  1.4 统计学处理 使用SPSS 11.0统计软件进行统计,数据以±s表示。计量资料采用t检验,计数资料采用χ2检验,P<0.05为差异有统计学意义。

  2 结 果

  2.1 组织病理学结果 假手术组胰腺组织肉眼及镜下结构无明显异常。胰腺炎组肉眼观胰腺明显肿胀,伴有出血坏死;镜下见间质血管扩张、淤血,灶状胰腺腺泡与脂肪坏死,大量炎性细胞浸润,红细胞渗出,胰腺细胞结构消失。治疗组肉眼病变及镜下观察组织结构破坏明显减轻。

  2.2 免疫组化测定 治疗组TNF-α、IL-1β含量显著低于胰腺炎组(P<0.01 ),见表1、2。表1 各组TNF-α的.D值表2 各组IL-1β的D值(n=8,±s)与假手术组比较:*P<0.01;与胰腺炎组比较:△P<0.01

  3 讨 论